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GYT Medium, Liquid
Catalog Number MM 009-01
Storage Temperature 2~8°C
GYT Medium, used in preparing electrocompetent cells. GYT Medium (glycerol-yeast extract-tryptone) Used resuspend the pellet of E. coli and frozen electrocompetent cells.
GYT medium should be stored at 2~8°C. Deterioration of GYT medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of GYP medium. Product label bears expiration date.
Biological Performance Characteristics
The electroporation capacity of GYT medium is tested in appropriate medium using an appropriate cell line(s). Harvest cells by centrifugation and resuspend the pellet in ice-cold GYT medium. Dilute the cell suspension to a concentration of 2x1010 to 3x1010 cells/mL with ice-cold GYT medium. Transfer 40ul of the suspension to an ice-cold electroporation cuvette and test whether arcing occurs when an electrical discharge is applied (electrical pulse of 25 uF capacitance, 2.5 kV, and 200 ohm). Transfer the cells incubate the cultures with gentle rotation for 1 hour at 37C. Plate different volumes of the electroporated cells onto SOB agar medium and the appropriate antibiotic.
For In Vitro Use Only