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HBSS - 10X (LB203-04)

HBSS - 10X (LB203-04)


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Size
Price
Quantity
500ML
₩40,000
1L
₩70,500
 
Special Order for bulk

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Product Details

제품 설명서 (PDF 파일 다운로드) MSDS (PDF 파일 다운로드)

 

Hanks’ Balanced Salt Solutions (HBSS), Liquid
Cell Culture Tested

Catalog Number LB 003-01 (1X), LB 203-03 (10X)
Catalog Number LB 003-02 (1X), LB 203-05 (10X)
Catalog Number LB 003-03 (1X), LB 203-04 (10X)
Catalog Number LB 003-04 (1X), LB 203-06 (10X)

Storage Temperature 15~30°C

Product Description
Buffered saline (BS) is one of the balanced salt solutions bearing the buffering capacity and capable of maintaining osmotic balance similar to the natural condition of body. Of many salines, phosphate-buffered saline (PBS), Dulbecco’s phosphate-buffered saline (D-PBS), Earle’s balanced salt solution (EBSS), and Hanks’ balanced salt solution (HBSS) are widely used because the buffering capacity of phosphate is effective at in vivo neutral pH with major roles in maintaining viability or integrity rather than promoting growth or functions of cells or molecules. Among these BSs, PBS and D-PBS contain the basic inorganic salts. Other principal functions of BSs are as follows; (1) dissolve or dilute materials for use in cell culture, (2) provide inorganic salts essential for the basic cell metabolism, (3) maintain the osmotic balance, (4) maintain pH with buffering capacity, and (5) eliminate certain components as serum or trypsin.

Storage/Stability
The buffered salines should be stored at 15~30°C. For long time storage, store at 2~8°C. Deterioration of the liquid may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the liquid. Product label bears expiration date.

Biological Performance Characteristics
The growth-maintaining capacity of HBSS is tested in an appropriate medium after storage of an appropriate cell line. Growth rates are examined through three subculture generations and compared with parallel cultures using the standardized control saline. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and final cell density are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.

Precautions
For In Vitro Use Only

Product Name Description Cat. No. Recommended Storage Temp. Size
Hanks’ Balanced Salt Solution (HBSS) (1X), liquid With phenol red
With calcium chloride
With magnesium sulfate
With sodium bicarbonate
LB 003-01 Store at 15-30°C 500ML, 1L
Hanks’ Balanced Salt Solution (HBSS) (1X), liquid Without phenol red
With calcium chloride
With magnesium sulfate
With sodium bicarbonate
LB 003-02 Store at 15-30°C 500ML, 1L
Hanks’ Balanced Salt Solution (HBSS) (1X), liquid With phenol red
With sodium bicarbonate
Without calcium chloride
Without magnesium sulfate
LB 003-03 Store at 15-30°C 500ML, 1L
Hanks’ Balanced Salt Solution (HBSS) (1X), liquid Without phenol red
With sodium bicarbonate
Without calcium chloride
Without magnesium sulfate
LB 003-04 Store at 15-30°C 500ML, 1L
Hanks’ Balanced Salt Solution (HBSS) (10X), liquid With phenol red
With calcium chloride
With magnesium sulfate
Without sodium bicarbonate
LB 203-03 Store at 15-30°C 500ML, 1L
Hanks’ Balanced Salt Solution (HBSS) (10X), liquid With phenol red
With sodium bicarbonate
Without calcium chloride
Without magnesium sulfate
LB 203-04 Store at 15-30°C 500ML, 1L
Hanks’ Balanced Salt Solution (HBSS) (10X), liquid With phenol red
With calcium chloride
With magnesium sulfate
Without sodium bicarbonate
LB 203-05 Store at 15-30°C 500ML, 1L
Hanks’ Balanced Salt Solution (HBSS) (10X), liquid Without phenol red
With calcium chloride
With magnesium sulfate
Without sodium bicarbonate
LB 203-06 Store at 15-30°C 500ML, 1L

* In effort to cater to the individual needs of our clients, Welgene provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH.

 

Comparison of HBSS

Cat. No. Calcium Chloride Magnesium Sulfate Phenol Red Sodium Bicarbonate Conc. Appearance
LB 003-01 1X liquid
LB 003-02 - 1X liquid
LB 003-03 - - 1X liquid
LB 003-04 - - - 1X liquid
LB 203-03 - 10X liquid
LB 203-04 - - 10X liquid
LB 203-05 - - - 10X liquid
LB 203-06 - - - - 10X liquid

* N/A, Not Applicable