[NOTICE]   신제품 보러가기
HEPES buffer solution(1.0M), (BB001-01)

HEPES buffer solution(1.0M), (BB001-01)

  오후 1시 이전 주문은 당일 발송됩니다.
  냉장, 냉동 제품은 금요일에는 발송이 불가능하며, 차주 월요일에 발송됩니다.
  재고 소진 시 배송이 지연 될 수 있습니다.(제품마다 생산소요기간 상이)
Special Order for bulk

Contact Us
Product Details

제품 설명서 (PDF 파일 다운로드) MSDS (PDF 파일 다운로드)


HEPES Buffer Solution (1.0 M)
Catalog Number BB 001-01

Storage Temperature 2~8°C 

Product Description
HEPES has been described as one of the best all-purpose buffers available for biological research. HEPES has been used in a wide variety of applications, including tissue culture. HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. Buffer strength for cell culture applications is usually in the range of 10 to 25 mM. But HEPES is not recommended for certain protein applications; it interferes with the Folin-Ciocalteu protein assay. A buffer solution of HEPES can be prepared by any of several methods. The free acid can be added to water, then titrated with approximately one-half mole equivalent of sodium hydroxide or potassium hydroxide to the precise pH desired, with adjustments made for final temperature and volume. Alternatively, equimolar concentrations of HEPES and of sodium HEPES can be mixed in approximately equal volumes, back-titrating with either solution to the appropriate pH.

BB 00101 contains 238.3 g/L HEPES in cell/tissue culture grade water (LS016-01).

The concentrated HEPES buffer should be stored at 2~8°C. Deterioration of the solution may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. Product label bears expiration date.


For In Vitro Use Only


Product Name Description Cat. No. Recommended Storage Temp. Size
HEPES Buffer Solution (1.0 M) Contains 238.3 g/L HEPES
in cell/tissue culture grade water
pH 7.2 - 7.5
BB 001-01 Store at 2-8°C 100ML