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Insulin Solution (10 mg/ml)
Human Recombinant from E. coli
Contains 10 mg/mL insulin in 10 mM HCl
Cell culture tested
Catalog Number LS 038-01
Storage Temperature -5~-20°C
Insulin is a protein hormone, produced and secreted from B cells of the islet of Langerhans. Insulin is composed of two polypeptide chains (A and B, total 51 amino acids) linked together by disulfide bridge. In vivo, insulin is involved in various actions, such as the cellular uptake, utilization and storage of glucose, amino acids, and fatty acids, and the inhibition of the breakdown of glycogen, protein, and fat. In vitro, insulin acts as a growth factor for many cells in serum-free medium, and used in serum-containing medium for some primary cell cultures.
LS 038-01 is produced from E. coli by recombination of human insulin DNA, which is chemically, physically and biologically identical to human pancreatic insulin. LS038-01 contains 10 mg/mL of insulin (human recombinant from E. coli) in 10 mM HCl. Generally, insulin is used at the concentration of 1~10 μg/mL. Add aseptically 0.1~1 mL of this solution to 1 L of an appropriate medium. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal) and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.
Insulin solution should be stored at -5~-20°C in the dark. Deterioration of the liquid may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pHchange. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.
Biological Performance Characteristics
The growth-promoting capacity of insulin solution is tested in a medium containing 10% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period, cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.
For In Vitro Use Only