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IPL-41 Insect Medium (1X), Liquid
With sodium bicarbonate
Insect cell culture tested
Catalog Number LM 502-01
Storage Temperature 2~8°C
IPL-41 Insect Medium was developed for a large scale culturing the cells derived from the fall armyworm, Spodoptera frugiperda at the USDA Insect Pathology Laboratory by Weiss et al. The growth and maintenance of lepidopteran cells and the propagation of viruses were conducted in this medium. Using spodoptera cells with baculovirus expression system in this medium, large scales of a recombinant protein expression were studied and reported.
LM 502-01 contains 1000 mg/L L-glutamine and 350 mg/L sodium bicarbonate. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal), and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.
The liquid medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.
Biological Performance Characteristics
The growth-promoting capacities of IPL-41 Medium are tested in a medium containing 10% FBS using an appropriate insect cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period, cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.
For In Vitro Use Only
* In effort to cater to the individual needs of our clients, WelGENE provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH.