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Leibovitz’s L-15 Medium (LM003-01)

Leibovitz’s L-15 Medium (LM003-01)


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Size
Price
Quantity
500ML
₩24,900
1L
₩42,800
 
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Product Details

제품 설명서 (PDF 파일 다운로드) MSDS (PDF 파일 다운로드)

 

Leibovitz’s L-15 Medium (1X), Liquid
With L-glutamine

Catalog Number LM 003-01
Storage Temperature 2~8°C

Product Description
Leibovitz’s L-15 is buffered by its complement of salts, free base amino acids and galactose substitute for glucose to help maintain physiological pH control. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses also have been successfully cultivated in this medium.

LM 003-01 contains 300 mg/L L-glutamine. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal) and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.

Storage/Stability
The liquid medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.

Biological Performance Characteristics
The growth-promoting capacities of Leibovitz’s L-15 medium are tested in a medium containing 10% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.

Precautions
For In Vitro Use Only

 

Product Name Description Cat. No. Recommended Storage Temp. Size
Leibovitz’s L-15 Medium (1X), liquid With L-glutamine LM 003-01 Store at 2-8°C 500ML, 1L





* In effort to cater to the individual needs of our clients, Welgene provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH.


Comparison of liquid Leibovitz’s L-15 Medium

Cat. No. L-Glutamine (mg/L) D-Glucose (mg/L) D-Galactose (mg/L) Phenol Red (mg/L) Sodium Bicarbonate (mg/L)
LM 003-01 300.00 (2.05 mM) - 900.00 10.00 -



Comparison of special ordered Leibovitz’s L-15 Medium

Cat. No. Basic Formulation Special Modification
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* In effort to cater to the individual needs of our clients, Welgene provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH.