Medium 199 (1X), Liquid
냉장, 냉동 제품은 금요일에는 발송이 불가능하며, 차주 월요일에 발송됩니다.
재고 소진 시 배송이 지연 될 수 있습니다.(제품마다 생산소요기간 상이)
Medium 199 (1X), Liquid
With Earle’s salts
With sodium bicarbonate
Catalog Number LM 006-01 (with L-glutamine)
LM 006-02 (without L-glutamine)
Storage Temperature 2~8°C
Product Description
Medium 199 contains various combinations of vitamins, amino acids and other factors. It has broad species applicability, particularly for cultivation of non-transformed cells. Morgan and his coworkers reported their efforts to produce a totally defined nutritional source, instead of serum, for cell cultures. However, it was found that long-term cultivation of cells required addition of a serum supplement to the culture. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.
LM 006-01 is based on the Earle’s balanced salts, and contains 100 mg/L of L-glutamine, 2200 mg/L of sodium bicarbonate. LM 006-02 is based on the Earle’s balanced salts, and contains 2200 mg/L of sodium bicarbonate but no L-glutamine. Add 3.42 ml of L-glutamine (LS 002-01, 200 mM) per 1 L of medium, if desired. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal), and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.
Storage/Stability
The liquid medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.
Biological Performance Characteristics
The growth-promoting capacities of Medium 199 are tested in a liquid medium using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.
|
Product Profile |
LM 006-01 |
LM 006-02 |
|
Appearance |
Red translucent solution |
Red translucent solution |
|
pH at RT |
7.0 ~ 7.6 |
7.0 ~ 7.6 |
|
Osmolality |
280 ~ 310 mOsm/kg H2O |
280 ~ 310 mOsm/kg H2O |
|
Endotoxin |
£ 1.0 EU/ml |
£ 1.0 EU/ml |
|
Sterility |
Sterilized by 0.2 mm filtration system. Sterility tests are performed in accordance with protocols described in USP. |
|
|
|
Salts |
L-glutamine |
HEPES |
Phenol red |
|
LM 006-01 |
Earle’s |
O |
- |
O |
|
LM 006-02 |
Earle’s |
- |
- |
O |
|
LM 006-03 |
Earle’s |
- |
O |
O |
|
LM 006-04 |
Earle’s |
O |
O |
O |
|
LM 006-05 |
Earle’s |
O |
O |
- |
|
LM 006-06 |
Hanks’ |
O |
- |
O |
|
LM 006-07 |
Hanks’ |
O |
O |
O |
Precautions
For In Vitro Use Only
|
Components |
LM 006-01 |
LM 006-02 |
|
CaCl2 (anhydrous) |
200.00 |
200.00 |
|
Fe(NO3)3·9H2O |
0.72 |
0.72 |
|
KCl |
400.00 |
400.00 |
|
MgSO4 (anhydrous) |
98.00 |
98.00 |
|
NaCl |
6800.00 |
6800.00 |
|
NaHCO3 |
2200.00 |
2200.00 |
|
NaH2PO4·H2O |
140.00 |
140.00 |
|
Adenine Sulfate |
10.00 |
10.00 |
|
Adenosine-5-triphosphate |
1.00 |
1.00 |
|
Adenosine-5-phosphate |
0.20 |
0.20 |
|
Chloesterol |
0.20 |
0.20 |
|
2-deoxy-D-ribose |
0.50 |
0.50 |
|
D-Glucose |
1000.00 |
1000.00 |
|
Glutathione (reduced) |
0.05 |
0.05 |
|
Guanine·HCl |
0.30 |
0.30 |
|
HEPES |
- |
- |
|
Hypoxanthine·Na |
0.40 |
0.40 |
|
Phenol Red |
20.00 |
20.00 |
|
Ribose |
0.50 |
0.50 |
|
Sodium Acetate |
50.00 |
50.00 |
|
Thymine |
0.30 |
0.30 |
|
Tween 80 |
20.00 |
20.00 |
|
Uracil |
0.30 |
0.30 |
|
Xanthine·Na |
0.340 |
0.340 |
|
L-Alanine |
25.00 |
25.00 |
|
L-Arginine·HCl |
70.00 |
70.00 |
|
L-Aspartic Acid |
30.00 |
30.00 |
|
L-Cystein·HCl·H2O |
0.10 |
0.10 |
|
L-Cysteine·2HCl |
26.00 |
26.00 |
|
Glycine |
50.00 |
50.00 |
|
L-Glutamic Acid |
75.00 |
75.00 |
|
L-Glutamine |
100.00 |
- |
|
L-Histidine·HCl·H2O |
22.00 |
22.00 |
|
L-Hydroxyproline |
10.00 |
10.00 |
|
L-Isoleucine |
40.00 |
40.00 |
|
L-Leucine |
60.00 |
60.00 |
|
L-Lysine·HCl |
70.00 |
70.00 |
|
L-Methionine |
15.00 |
15.00 |
|
L-Phenylalanine |
25.00 |
25.00 |
|
L-Proline |
40.00 |
40.00 |
|
L-Serine |
25.00 |
25.00 |
|
L-Threonine |
30.00 |
30.00 |
|
L-Tryptophan |
10.00 |
10.00 |
|
L-Tyrosine·2Na·2H2O |
58.00 |
58.00 |
|
L-Valine |
25.00 |
25.00 |
|
Ascorbic Acid |
0.050 |
0.050 |
|
α –Tocopherol Phosphate (sodium salt) |
0.01 |
0.01 |
|
Biotin |
0.01 |
0.01 |
|
Calciferol |
0.10 |
0.10 |
|
D-Ca Pantothenate |
0.01 |
0.01 |
|
Choline Chloride |
0.50 |
0.50 |
|
Folic Acid |
0.01 |
0.01 |
|
i-Inositol |
0.05 |
0.05 |
|
Menadione (sodium bisulfite) |
0.01 |
0.01 |
|
Niacin |
0.025 |
0.025 |
|
Niacinamide |
0.025 |
0.025 |
|
Para-aminobenzoic Acid |
0.05 |
0.05 |
|
Pyridoxal·HCl |
0.025 |
0.025 |
|
Pyridoxine·HCl |
0.025 |
0.025 |
|
Riboflavin |
0.01 |
0.01 |
|
Thiamine·HCl |
0.01 |
0.01 |
|
Vitamin A (acetate) |
0.14 |
0.14 |
References
Morgan, J.F, Morton, H.J. and Parker, R.C. (1950). The Nutrition of Animal Cells in Tissue Culture. I. Initial Studies on a Synthetic Medium. Proc. Soc. Exp. Biol. Med. 73, 1-8.
Morgan, J.F., Campbell, E. and Morton, H.J. (1955). The Nutrition of Animal Tissues Cultivated In Vitro. I. A Survey of Natural Materials as Supplements to Synthetic Medium. J.N.C.I. 16:2, 557-567.
Morton, H.J. (1970). A Survey of Commercially Available Tissue Culture Media. In Vitro. 6, 89-108.
Rutzky, L.P. and Pumper, R.W., (1974). Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro. 9, 468-469.