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Minimum Essential Medium Eagle (LM007-14)

Minimum Essential Medium Eagle (LM007-14)


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Size
Price
Quantity
500ML
₩20,800
1L
₩35,900
 
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Product Details

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Minimum Essential Medium Eagle (MEM), Liquid
With Earle’s salts

Catalog Number 
LM 007-12 (without L-glutamine and phenol red)
LM 007-13 (with 10 mM HEPES and L-glutamine)
LM 007-14 (with 1500 mg/L sodium bicarbonate and without L-glutamine)
Storage Temperature 2~8°C

Product Description
Minimum Essential Medium (MEM) was developed by Harry Eagle and has been used for wide variety of cells grown in monolayer. Basal Medium Eagle (BME), previously developed, could not meet the specific nutritional requirements for cultivating normal mammalian fibroblasts and certain subtypes of HeLa cells. MEM includes high concentration of amino acids so that it can more closely approximate the protein composition of cultured mammalian cells. The formulation of MEM containing either Hanks’ or Earle’s salts can be supplemented by optional non-essential amino acids for the variable types of cells. The formulation can be further modified by optional elimination of calcium, necessary for attachment, to permit growth of cells in suspension culture.

LM 007-12 is based on the Earle’s balanced salts, and does not contain L-glutamine and phenol red. LM 007-13 is based on the Earle’s balanced salts, and contains 10 mM HEPES and 292 mg/L L-glutamine. LM 007-14 is based on the Earle’s balanced salts, and contains 1500 mg/L sodium bicarbonate, but no L-glutamine. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal), and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.

Storage/Stability
The liquid medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.

Biological Performance Characteristics
The growth-promoting capacities of MEM are tested in a medium containing 5~10% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.

Precautions
For In Vitro Use Only


Product Name Description Cat. No. Recommended Storage Temp. Size
Minimum Essential Medium Eagle (MEM) (1X), liquid With Earle’s salts
With L-glutamine
LM 007-01 Store at 2-8°C 500ML, 1L
Minimum Essential Medium Eagle (MEM), Joklik Modification (1X), liquid Modified for suspension cultures
With L-glutamine
With 10X phosphate
Without calcium chloride
LM 007-02 Store at 2-8°C 500ML, 1L
Minimum Essential Medium Eagle, Spinner Modification (S-MEM) (1X), liquid Modified for suspension cultures
With Earle’s salts
With L-glutamine
LM 007-03 Store at 2-8°C 500ML, 1L
Minimum Essential Medium Eagle (MEM) (1X), liquid With Hanks’ salts
With L-glutamine
LM 007-05 Store at 2-8°C 500ML, 1L
Minimum Essential Medium Eagle (MEM) (1X), liquid With Hanks’ salts
With L-glutamine
With 25 mM HEPES
LM 007-06 Store at 2-8°C 500ML
Minimum Essential Medium Eagle (MEM) (1X), liquid With Earle’s salts
With non-essential amino acids
With L-glutamine
With L-glutamine
LM 007-07 Store at 2-8°C 500ML, 1L
Minimum Essential Medium Eagle (MEM) (1X), liquid With Earle’s salts
With non-essential amino acids
Without L-glutamine
LM 007-08 Store at 2-8°C 500ML
Minimum Essential Medium Eagle (MEM) (1X), liquid With Earle’s salts
With 25 mM HEPES
With L-glutamine
LM 007-09 Store at 2-8°C 500ML, 1L
Minimum Essential Medium Eagle (MEM) (1X), liquid With Earle’s salts
With 25 mM HEPES
Without L-glutamine
LM 007-10 Store at 2-8°C 500ML, 1L
Minimum Essential Medium Eagle (MEM) (1X), liquid With Earle’s salts
Without L-glutamine
LM 007-11 Store at 2-8°C 500ML, 1L
Minimum Essential Medium Eagle (MEM) (1X), liquid With Earle’s salts
Without L-glutamine
Without phenol red
LM 007-12 Store at 2-8°C 500ML
Minimum Essential Medium Eagle (MEM) (1X), liquid With Earle’s salts
With 10 mM HEPES
With L-glutamine
LM 007-13 Store at 2-8°C 500ML, 1L
Minimum Essential Medium Eagle (MEM) (1X), liquid With Earle’s salts
With 1500 mg/L sodium bicarbonate
Without L-glutamine
LM 007-14 Store at 2-8°C 500ML, 1L

* In effort to cater to the individual needs of our clients, Welgene provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH.

 

Comparison of liquid MEM 

Cat. No. Balanced Salt Solution L-Glutamine
(mg/L)
HEPES
(mg/L)
CaCl2 (anhyd.)
(mg/L)
Phenol Red
(mg/L)
L-Methionine
(mg/L)
NEAAs
(mg/L)
Sodium Bicarbonate
(mg/L)
LM 007-01 Earle's Salts 292.00 (2 mM) - 200.00 10.00 15.00 - 2200.00
LM 007-02 Joklik modification 292.00 (2 mM) - - 10.00 15.00 - 2200.00
LM 007-03 Spinner modification 292.00 (2 mM) - - 10.00 15.00 - 2200.00
LM 007-05 Hanks' Salts 292.00 (2 mM) - 140.00 10.00 15.00 - 350.00
LM 007-06 Hanks' Salts 292.00 (2 mM) 5958.00 (25 mM) 140.00 10.00 15.00 - 350.00
LM 007-07 Earle's Salts 292.00 (2 mM) - 200.00 10.00 15.00 0.1 mM 2200.00
LM 007-08 Earle's Salts - - 200.00 10.00 15.00 0.1 mM 2200.00
LM 007-09 Earle's Salts 292.00 (2 mM) 5958.00 (25 mM) 200.00 10.00 15.00 - 2200.00
LM 007-10 Earle's Salts - 5958.00 (25 mM) 200.00 10.00 15.00 - 2200.00
LM 007-11 Earle's Salts - - 200.00 10.00 15.00 - 2200.00
LM 007-12 Earle's Salts - - 200.00 - 15.00 - 2200.00
LM 007-13 Earle's Salts 292.00 (2 mM) 2383.20 (10 mM) 200.00 10.00 15.00 - 2200.00
LM 007-14 Earle's Salts - - 200.00 10.00 15.00 - 1500.00

* NEAAs, Non-essential amino acids (L-Ala, L-Asn, L-Asp, L-Glu, Gly, L-Pro, and L-Ser)

 

Comparison of special ordered liquid MEM

Cat. No. Basic Formulation Special Modification
LM 007-04 LM 007-01 Without L-Methionine
LM 007-50 * Sterilized by autoclaving
Without sodium bicarbonate
LM 007-51 LM 007-03 With non-essential amino acids
LM 007-54 LM 007-07 With Sodium pyruvate 1mM, with Sodium bicarbonate 1,500 mg/L
LM 007-60 LM 007-07 Without D-glucose
LM 007-62 LM 007-01 With D-Valine and without L-Valine

* In effort to cater to the individual needs of our clients, Welgene provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH.