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Miller's LB Broth (MM002-01)

Miller's LB Broth (MM002-01)

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LB (Luria-Bertani) Medium
(Miller’s LB Medium), Liquid

Catalog Number MM 002-01
Storage Temperature 2~8°C

Product Description
Luria-Bertani medium (LB), a nutritionally rich medium, is primarily used for the growth of bacteria. LB media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins. It continues to be one of the most common media used for maintaining and cultivating laboratory recombinant strains of Escherichia coli.

LB medium should be stored at 2~8°C. Deterioration of LB medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. Product label bears expiration date.

Biological Performance Characteristics
LB medium contained tryptone, yeast extract and sodium chloride. Peptides and peptones are provided by tryptone. Vitamins and certain trace elements are provided by yeast extract. Sodium ions for transport and osmotic balance are provided by sodium chloride. Tryptone is used to provide essential amino acids to the growing bacteria, while the yeast extract is used to provide a plethora of organic compounds helpful for bacterial growth. LB medium tested bacteria culture.

For In Vitro Use Only


Components MM 002-01 (g/L)
Tryptone 10.0
Yeast extract 5.0
Sodium chloride 10.0

Product Name Description Cat. No. Recommended Storage Temp. Size
LB Medium (Miller’s LB Medium), liquid Components
  10 g/L Tryptone
  5 g/L Yeast Extract
  10 g/L NaCl
pH 7.0
Autoclaved (121°C, 15 lb/sq. in., 20 min)
Bacterial culture tested
MM 002-01 Store at 2-8°C 500ML, 1L