Prestained Protein Size Marker
Catalog Number PM 100-01
Storage Temperature -20°C
Prestained Protein Size Marker is a prestained marker for a
SDS-PAGE analysis. Well defined 10 recombinant proteins
are included and supplied in a ready-to-use formula (no need
to boil before use). It is suitable for sizing proteins between 9
and 170 kDa by SDS-PAGE.
Chromophores are covalently linked to proteins in prestained
size marker, and 10 prestained proteins are visible during
electrophoresis or electrophoretic transfer from the gel to a
membrane. The 75 kDa protein labeled with orange color
plays a ruler function for easy identification of banding.
Because coupling of chromophores to the proteins in
pre-stained marker affects their apparent molecular weights in
SDS-PAGE relative to unstained proteins, unstained protein
markers should be used for accuracy >95%.
Prestained Protein Size Marker should be stored immediately
upon receipt at -20
oC. If stored in the recommended
temperature, this product will be stable until the expiration date
printed out on the label.
Recommended loading volume
- 5 l/lane for a mini-gel (10 X 8 cm2
, 0.75 or 1 mm thick)
- 10-15 l/lane for a large-gel (30 X 20 cm2
, 1 or 1.5 mm thick)
- 2-5 l/lane for a western transfer onto PVDF or NC membranes
- Repeated freezing & thawing, long-term storage and re-use
of pipette tip may degrade proteins rapidly.
- The marker is provided as a ready to load format, meaning
that there is no need to boil before use. But precipitation of
SDS may occur by long-term storage at below 4oC, and you
can load marker just after dissolving the precipitant by
raising to 50-80oC for a few minutes.
- The slight size difference can be seen in the course of
manufacturing. Please confirm the batch numbers
Fig A. Electrophoresis pattern in 15% SDS-PAGE gel Fig B. Molecular weight and color of individual prestained protein