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RPMI 1640 Medium (2X), Liquid
Without sodium bicarbonate
Catalog Number LM 204-01 (With Phenol red)
Catalog Number LM 204-51 (Without Phenol red)
Storage Temperature 2~8°C
For long-term culture of blood cells, RPMI 1640 medium was developed by Moore et al., at Rosewell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI 1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI 1640 medium is a modification of McCoy’s 5A medium and it has wide applicability for supporting growth of many types of cells, especially suspension cells such as normal and neoplastic leukocytes.
LM 204-01 contains 600 mg/L of L-glutamine but no sodium bicarbonate. LM 204-51 contains 600 mg/L of L-glutamine but no phenol red and sodium bicarbonate. Add 26.67 ml of 7.5% sodium bicarbonate (BB 002-01) per 1 L of 1X medium. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal), and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.
The liquid medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.
Biological Performance Characteristics
The growth-promoting capacities of the liquid media are tested in a medium containing 10% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiencies, doubling time, and final cell densities are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity. Test results are available upon request.
Preparation Procedure (1 L of 1X medium)
- Mix 500 ml of 2X RPMI 1640 Medium and 400 ml of sterile cell/tissue culture grade water (LS 016-01) in an appropriate vessel.
- Add 26.67 ml of sodium bicarbonate solution (7.5%, BB 002-01) and mix well.
- If the desired pH is different from the pH in table below, adjust pH using sterile 1 N HCl (LS 003-02) or 1 N NaOH (LS 012-02).
- Fill up to 1 L by adding sterile cell/tissue culture grade water.
For In Vitro Use Only