RPMI 1640 Vitamins (LS010-01)
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RPMI 1640 Vitamin Solution (100X)
Sterile-filtered
Endotoxin tested
Cell culture tested
Catalog Number LS 010-01
Storage Temperature -5~-20°C
Product Description
Adding supplements of vitamins to media both stimulates growth and prolongs the viability of cells in culture. This concentrated supplement adds nutrients and reduces the biosynthetic burden on cell in vitro.
LS 010-01 contains D-biotin, choline chloride, folic acid, i-inositol, niacinamide, p-amino benzoic acid, D-pantothenate, pyridoxine, riboflavin, thiamine, and vitamin B12 in cell/tissue culture grade water (LS 016-01) with 0.85% NaCl.
Storage/Stability
The RPMI 1640 vitamin solution should be stored at -5~-20°C in the dark. Deterioration of the liquid may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.
Biological Performance Characteristics
The growth-maintaining capacity of RPMI 1640 vitamin solution is tested in RPMI 1640 containing 5% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period, cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.
Precaution
For In Vitro Use Only
| Componentes | LS 010-01 |
|---|---|
| D-Biotin | 20.00 |
| Choline Chloride | 300.00 |
| Folic Acid | 100.00 |
| i-Inositol | 3500.00 |
| Niacinamide | 100.00 |
| p-Amino Benzoic Acid | 100.00 |
| D-Pantothenic Acid-1/2 Ca | 25.00 |
| Pyridoxine·HCl | 100.00 |
| Riboflavin | 20.00 |
| Thiamine·HCl | 100.00 |
| Vitamin B12 | 5.00 |
| NaCl | 8500.00 |
| Product Name | Description | Cat. No. | Recommended Storage Temp. |
Size |
|---|---|---|---|---|
| Peptone Solution (5%) | Contains 50.0 g/L peptone in cell/tissue culture grade water Sterile-filtered Cell culture tested Endotoxin tested |
LS 032-01 | Store at 2-8°C | 100ML |
| Poloxamer 188 Solution (10%) | Contains 100.0 g/L Poloxamer 188 in cell/tissue culture grade water Sterile-filtered Insect cell culture tested Endotoxin tested Poloxamer188 is equivalent to Pluronic® F-68. Pluronic® F-68 is a registered trademark of BASF |
LS 105-02 | Store at 15-30°C | 100ML |
| RPMI 1640 Amino Acid Solution (50X) | Prepared in cell/tissue culture grade water Without L-glutamine Sterile-filtered Cell culture tested Endotoxin tested |
LS 009-01 | Store at 2-8°C | 100ML |
| RPMI 1640 Vitamin Solution (100X) | Prepared in 0.85% NaCl Sterile-filtered Cell culture tested Endotoxin tested |
LS 010-01 | Store at less than 0°C | 100ML |
| Sodium Butyrate Solution (1.0 M) | Contains 110.1 g/L sodium butyrate in cell/tissue culture grade water Sterile-filtered Cell culture tested Endotoxin tested |
LS 033-01 | Store at 2-8°C | 100ML |
| Sodium Propionate Solution (2.0 M) | Contains 192.1 g/L sodium propionate in cell/tissue culture grade water Sterile-filtered Cell culture tested Endotoxin tested |
LS 036-01 | Store at 2-8°C | 100ML |
| Sodium Pyruvate Solution (100 mM) | Contains 11.0 g/L sodium pyruvate in cell/tissue culture grade water Sterile-filtered Cell culture tested Endotoxin tested |
LS 013-01 | Store at 2-8°C | 100ML |
| Soy Protein Solution (5%) | Contains 50.0 g/L soy protein in cell/tissue culture grade water Sterile-filtered Cell culture tested Endotoxin tested |
LS 031-01 | Store at 2-8°C | 100ML |