Sodium Pyruvate 100mM (LS013-01)
냉장, 냉동 제품은 금요일에는 발송이 불가능하며, 차주 월요일에 발송됩니다.
재고 소진 시 배송이 지연 될 수 있습니다.(제품마다 생산소요기간 상이)
| 제품 설명서 (PDF 파일 다운로드) | MSDS (PDF 파일 다운로드) | |
Sodium Pyruvate Solution(100 mM)
Contains 11.0 mg/mL sodium pyruvate
in cell/tissue culture grade water
Sterile-filtered
Endotoxin tested
Cell culture tested
Catalog Number LS 013-01
Storage Temperature 2~8°C
Product Description
In aerobic condition, glucose is completely oxidized to CO2 by eukaryotic cells. In this process, pyruvate formed during glycolysis is transported to the mitochondria, where it is oxidized to CO2. These reactions of oxidation in mitochondria generate the biological energies in forms of ATP and GTP. For this reason, energy sources like glucose and pyruvate are generally supplemented to cell culture media.
LS013-01 contains 11.0 mg/mL of sodium pyruvate in tissue culture grade water. Generally, sodium pyruvate is used at the concentration of 1 mM (110 mg/L). Add 5 mL of this solution to 500 mL of an appropriate medium, then 1 mM (110 mg/L) of sodium pyruvate mediums is prepared. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal) and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.
Storage/Stability
The liquid should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. Product label bears expiration date.
Biological Performance Characteristics
The growth-promoting capacity of sodium pyruvate solution is tested in a medium containing 10% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and final cell densities are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.
Precautions
For In Vitro Use Only
Molecular Weight
110 g/mole
Molecular Formula
C3H3O3Na
Molecular Structure
| Product Name | Description | Cat. No. | Recommended Storage Temp. |
Size |
|---|---|---|---|---|
| Peptone Solution (5%) | Contains 50.0 g/L peptone in cell/tissue culture grade water Sterile-filtered Cell culture tested Endotoxin tested |
LS 032-01 | Store at 2-8°C | 100ML |
| Poloxamer 188 Solution (10%) | Contains 100.0 g/L Poloxamer 188 in cell/tissue culture grade water Sterile-filtered Insect cell culture tested Endotoxin tested Poloxamer188 is equivalent to Pluronic® F-68. Pluronic® F-68 is a registered trademark of BASF |
LS 105-02 | Store at 15-30°C | 100ML |
| RPMI 1640 Amino Acid Solution (50X) | Prepared in cell/tissue culture grade water Without L-glutamine Sterile-filtered Cell culture tested Endotoxin tested |
LS 009-01 | Store at 2-8°C | 100ML |
| RPMI 1640 Vitamin Solution (100X) | Prepared in 0.85% NaCl Sterile-filtered Cell culture tested Endotoxin tested |
LS 010-01 | Store at less than 0°C | 100ML |
| Sodium Butyrate Solution (1.0 M) | Contains 110.1 g/L sodium butyrate in cell/tissue culture grade water Sterile-filtered Cell culture tested Endotoxin tested |
LS 033-01 | Store at 2-8°C | 100ML |
| Sodium Propionate Solution (2.0 M) | Contains 192.1 g/L sodium propionate in cell/tissue culture grade water Sterile-filtered Cell culture tested Endotoxin tested |
LS 036-01 | Store at 2-8°C | 100ML |
| Sodium Pyruvate Solution (100 mM) | Contains 11.0 g/L sodium pyruvate in cell/tissue culture grade water Sterile-filtered Cell culture tested Endotoxin tested |
LS 013-01 | Store at 2-8°C | 100ML |
| Soy Protein Solution (5%) | Contains 50.0 g/L soy protein in cell/tissue culture grade water Sterile-filtered Cell culture tested Endotoxin tested |
LS 031-01 | Store at 2-8°C | 100ML |