Tetracycline (10 mg/ml), (ML003-07)

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Product Details

Tetracycline, Hydrochloride Solution
Contains 10 mg/mL tetracycline, hydrochloride
in Ultra Pure Water
DNase, RNase and protease – none detected

Catalog Number ML 003-07
Storage Temperature -5~-20°C

Product Description
Tetracyclines possess a wide range of antimicrobial activity against gram-positive and gram-negative bacteria. The bacterial ribosome is the site of action of tetracyclines. Access to the ribosomes of gramnegative bacteria is obtained by passive diffusion through hydrophilic pores in the outer cell membrane and then by an energy-dependent active transport system that pumps all tetracyclines through the inner cytoplasmic membrane. This active transport system may require a periplasmic protein carrier. Tetracyclines bind specifically to 30S ribosomes and appear to inhibit protein synthesis by preventing access of aminoacyl tRNA to the acceptor site on the mRNA-ribosome complex. The inhibitory effects of the tetracyclines can be reversed by washing. This suggests that the reversibly bound antibiotic rather than the small portion of irreversibly bound drug is responsible for the antibacterial action.

ML 003-07 contains 10 mg/mL tetracycline, hydrochloride in Ultra Pure Water (ML 019-02). Working concentration is 10 ~ 20 μg/mL.

Storage/Stability
Tetracycline solution should be stored at -5~-20°C. Deterioration of the liquid may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. Product label bears expiration date.

Precautions
For In Vitro Use Only

Molecular Weight
480.9 g/mole

Molecular Formula
C₂₂H₂₈N₂O₈

Molecular Structure

Product Name	Description	Cat. No	Recommended Storage Temp.	Size
Ampicillin, Sodium Salt Solution (50 mg/ml)	C₁₆H₁₈N₃O₄SNa, FW 371.4 Ampicillin inhibits cell wall biosynthesis by inhibiting peptidoglycan cross-linking and is bactericidal only to growing cells Contains 50 mg/ml ampicillin, sodium salt in Ultra Pure Water Working concentration is 20 - 50 µg/ml Sterile-filtered DNase, RNase and protease-none detected	ML 003-01	Store at less than 0°C	1ML x 12 20ML 100ML
Chloramphenicol Solution (10 mg/ml)	C₁₁H₁₂Cl₂N₂O₅, FW 323.1 Chloramphenicol inhibits protein synthesis (elongation) by inhibiting translation on the 50S ribosomal subunit at the peptidyltransferase step Contains 10 mg/ml chloramphenicol in absolute ethanol Working concentration is 10 - 20 µg/ml Sterile-filtered DNase, RNase and protease-none detected	ML 003-02	Store at less than 0°C	1ML x 12 20ML 100ML
Gentamicin, Sulfate Salt Solution (10 mg/ml)	Gentamicin inhibits protein synthesis by binding to L6 protein of 50S ribosomal subunit Contains 10 mg/ml gentamicin, sulfate salt in Ultra Pure Water Working concentration is 10 - 15 µg/ml Sterile-filtered DNase, RNase and protease-none detected	ML 003-03	Store at less than 0°C	1ML x 12 20ML 100ML
Kanamycin, Sulfate Salt Solution (10 mg/ml)	C₁₈H₃₆N₄O₁₁, FW 582.6 Kanamycin inhibits protein synthesis by binding to 70S ribosomal subunit, inhibiting translocation, and the elicitation of miscoding Contains 10 mg/ml kanamycin, sulfate salt in Ultra Pure Water Working concentration is 25 - 50 µg/ml Sterile-filtered DNase, RNase and protease-none detected	ML 003-04	Store at less than 0°C	1ML x 12 20ML 100ML
Rifampicin (Rifamycin AMP) Solution (25 mg/ml)	C₄₃H₅₈N₄O₁₂, FW 823.0 Rifampicin inhibits initiation of RNA synthesis by binding to β-subunit of RNA polymerase Contains 25 mg/ml rifampicin in methanol Working concentration is 150 µg/ml Sterile-filtered DNase, RNase and protease-none detected	ML 003-05	Store at less than 0°C	20ML
Streptomycin, Sulfate Salt Solution (50 mg/ml)	C₄₂H₅₈N₄O₃S₃, FW 1457.4 Streptomycin inhibits protein synthesis by binding to S12 protein of 30S ribosomal subunit, causing misreading or inhibiting initiation Contains 50 mg/ml streptomycin, sulfate salt in Ultra Pure Water Working concentration is 25 - 50 µg/ml Sterile-filtered DNase, RNase and protease-none detected	ML 003-06	Store at less than 0°C	1ML x 12 20ML 100ML
Tetracycline, Hydrochloride Solution (10 mg/ml)	C₂₂H₂₈N₂O₈, FW 480.9 Tetracycline inhibits protein synthesis (elongation) by preventing binding of aminoacyl-tRNA to ribosome Contains 10 mg/ml tetracycline, hydrochloride in Ultra Pure Water Working concentration is 10 - 20 µg/ml Sterile-filtered DNase, RNase and protease-none detected	ML 003-07	Store at less than 0°C	1ML x 12